Laravel, api call slow first time (repeated call after 5s)

I work on a Vagrant+Puppet LAMP (2x cores, 2.5GB) stack where I develop my Laravel ver.8 based API. There is a default setup, JWT middleware and almost bare minimum (also try with a bare fresh install with same behaviour). After the first call, the response time is about 6 seconds. If I call the same API endpoint, the response time is 200ms. Each further call within 3 seconds interval is quick. If I pause the calls for > 3s and then call again, the response time is again slow at about 6s.

Attempts I made to solve this:

php artisan cache:clear
composer dump-autoload -a --apcu
php artisan config:cache
php artisan session:table
php artisan migrate

.env:
APP_DEBUG=false
SESSION_DRIVER=database
SESSION_LIFETIME=120
CACHE_DRIVER=memcached
MEMCACHED_HOST=127.0.0.1
MEMCACHED_PORT=11217

BTW, the api call returns only: return response()->json(['tst']); When I output phpinfo(); from index.php, the response time is each time (pausing to) about 70ms.

Also try to debug the calls with CacheGrind via Xdebug: XDEBUG_PROFILE=1. Here are the profiling results: https://filebin.net/izs3qm0gm3gt8jq6

I just mentioned that the autoloader takes longer but could not compare the results. Is there any way to figure out the issue?

Thank you in advance.

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